The current EHEC O104:H4, causing a severe outbreak in Germany (May 2011), is microbiologically characterized as follows (1) (27.5.2011; updated 30.5.2011):

Shigatoxin 1: – (negative), Shigatoxin 2 (vtx2a): + (positive), Intimin (eae): – (negative), Enterohemolysin: – (negative)

EaggEC Virulence plasmid:  –   aatA-PCR: + (positive),  –   aggR-PCR: + (positive),  –   aap-PCR: + (positive)

MLST Sequence Type:  ST678 (adk 6, fumC6, gyrB 5, icd 136, mdh 9, purA 7, recA 7). (**)

(**) MLST: kindly provided by Prof. Dr. H. Karch; Konsiliarlaboratorium für Hämolytisch-Urämisches Syndrom (HUS) Institut für Hygiene am Universitätsklinikum Münster Robert-Koch-Str. 41, 48149 Münster

Ampicillin  R

Amoxicillin/Clavulanic acid  R

Piperacillin/Sulbactam  R

Piperacillin/Tazobactam  R*

Cefuroxim  R

Cefuroxim-Axetil  R

Cefoxitin  R

Cefotaxim  R

Cetfazidim  R

Cefpodoxim  R

Imipenem  S

Meropenem  S

Amikacin  S

Gentamicin  S

Kanamycin  S

Tobramycin  S

Streptomycin  R

Nalidixinsäure  R

Ciprofloxacin  S

Norfloxacin  S

Tetracyclin  R

Nitrofurantoin  S

Trimethoprim/Sulfamethoxazol  R

Chloramphenicol  S

Fosfomycin  S

* defined as resistent (AES VITEK)

ESBL: + (CTX-M (Group 1), Other ß-Lactamases: TEM-Type +

Macrorestriction pattern (XbaI) from human E. coli O104:H4 isolates from the current outbreak

Screen shot 2011-05-31 at 8.23.05 PM.png

PFGE according to Prager et al. (2011) IJMM 301:181-191.

Lanes 1 and 5 MW-Standard Salmonella Braenderup H9812. Lane 2: RKI-11-02027 (HUS), Lane 3: RKI-11-02034 (diarrhea), Lane 4: RKI-11-02060 (bloody diarrhea).

1) Source: Nationales Referenzzentrum für Salmonellen und andere bakterielle Enteritiserreger; Robert Koch-Institut; Abteilung 1 Infektionskrankheiten