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      <title>Marler Blog - E. coli Outbreaks still a Risk in Leafy Greens - Comments</title>
      <link>http://www.marlerblog.com/</link>
      <description>Food Poisoning Lawyer &amp; Attorney : Bill Marler : Marler Clark</description>
      <language>en</language>
      <copyright>Copyright 2012</copyright>
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         <title>Rebecca T. of HonestMeat</title>
         <description><![CDATA[<p>I've never heard of E. coli cells reconstituting back into a living bacterium.  I actually was unaware that living things could do that.  All my years of biology did not teach me about such a phenomena.  Might you have a scientific reference from that conference that talks about this phenomena occurring in compost?<br />
Also, when you refer to "flies and other insects" excreting E. coli in their feces, which other insects?  Which strain of E.coli?</p>]]></description>
         <link>http://www.marlerblog.com/lawyer-oped/e-coli-outbreaks-still-a-risk-in-leafy-greens/#8315</link>
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         <category domain="http://www.marlerblog.com/">Lawyer Op-Ed</category>
         <pubDate>Fri, 12 Sep 2008 08:19:54 -0800</pubDate>
         <author>marler@marlerclark.com (Food Poisoning Attorney)</author>
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         <title>Bill Marler</title>
         <description><![CDATA[<p>"I've never heard of E. coli cells reconstituting back into a living bacterium. I actually was unaware that living things could do that. All my years of biology did not teach me about such a phenomena. Might you have a scientific reference from that conference that talks about this phenomena occurring in compost?"</p>

<p>The data is not yet published, but the phenomena has been described in foods and other samples.  The bacteria is not actually dead, but in a "dormant" state metabolically.</p>

<p>1.  Viable but non-culturable bacteria: their impact on public health</p>

<p><a href="http://www.ias.ac.in/currsci/nov252005/1650.pdf" rel="nofollow"><a href="http://www.ias.ac.in/currsci/nov252005/1650.pdf" rel="nofollow"><a href="http://www.ias.ac.in/currsci/nov252005/1650.pdf" rel="nofollow">http://www.ias.ac.in/currsci/nov252005/1650.pdf</a></a></a></p>

<p>"The viability of a bacterial cell was traditionally determined by its ability to grow and produce colonies.However, in recent years, many studies have revealed the ability of both Gram-positive and Gram-negative bacteria to go into a viable but non-culturable (VBNC) state. In this state, bacteria are still viable and show metabolic activity<br />
and respiration, but cannot be shown as colony forming units by the conventional plate counts and hence remain hidden1."</p>

<p>2.  Viable but non-culturable forms of food and waterborne bacteria: Quo Vadis? </p>

<p><a href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VHY-4CVX484-5&_user=4421&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=4421&md5=70669e8045ab0219a3e5b0cb2b8b4664" rel="nofollow"><a href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VHY-4CVX484-5&_user=4421&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=4421&md5=70669e8045ab0219a3e5b0cb2b8b4664" rel="nofollow"><a href="http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VHY-4CVX484-5&_user=4421&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=4421&md5=70669e8045ab0219a3e5b0cb2b8b4664" rel="nofollow">http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VHY-4CVX484-5&_user=4421&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=4421&md5=70669e8045ab0219a3e5b0cb2b8b4664</a></a></a></p>

<p>There is increasing evidence for a viable but non-culturable (VBNC) state in microbes, particularly in the stressing environment presented by modern foods with their varied pre-treatment and packaging strategies. This is a cause for concern because of evidence that microbial pathogens in such a state may retain their capacity to cause infections after ingestion by the consumer despite their inability to grow under the conditions employed in procedures for determining their presence in foods. Heavily stressed pathogenic species of bacteria in a VBNC or not immediately culturable state are potentially dangerous public health problems, particularly as stressed cells may be more virulent than well-fed bacteria. In this viewpoint article, I wish to focus on possible procedures for detecting such organisms and assessing their physiological state.</p>

<p>"Also, when you refer to "flies and other insects" excreting E. coli in their feces, which other insects? Which strain of E.coli?"</p>

<p>They looked at filth flies, blow flies and a couple other species.  The preliminary positive results were mostly from filth flies trapped in the fields.  They said additional studies are underway to identify the strains of E. coli excreted by the flies.</p>]]></description>
         <link>http://www.marlerblog.com/lawyer-oped/e-coli-outbreaks-still-a-risk-in-leafy-greens/#8316</link>
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         <category domain="http://www.marlerblog.com/">Lawyer Op-Ed</category>
         <pubDate>Fri, 12 Sep 2008 08:19:54 -0800</pubDate>
         <author>marler@marlerclark.com (Food Poisoning Attorney)</author>
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         <title>Sam K.</title>
         <description><![CDATA[<p>Gah!  Regarding flies:  <a href="http://www.ajtmh.org/cgi/reprint/61/4/625.pdf" rel="nofollow"><a href="http://www.ajtmh.org/cgi/reprint/61/4/625.pdf" rel="nofollow"><a href="http://www.ajtmh.org/cgi/reprint/61/4/625.pdf" rel="nofollow">http://www.ajtmh.org/cgi/reprint/61/4/625.pdf</a></a></a></p>]]></description>
         <link>http://www.marlerblog.com/lawyer-oped/e-coli-outbreaks-still-a-risk-in-leafy-greens/#8317</link>
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         <category domain="http://www.marlerblog.com/">Lawyer Op-Ed</category>
         <pubDate>Fri, 12 Sep 2008 08:19:54 -0800</pubDate>
         <author>marler@marlerclark.com (Food Poisoning Attorney)</author>
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