The current EHEC O104:H4, causing a severe outbreak in Germany (May 2011), is microbiologically characterized as follows (1) (27.5.2011; updated 30.5.2011):
Shigatoxin 1: – (negative), Shigatoxin 2 (vtx2a): + (positive), Intimin (eae): – (negative), Enterohemolysin: – (negative)
EaggEC Virulence plasmid: – aatA-PCR: + (positive), – aggR-PCR: + (positive), – aap-PCR: + (positive)
MLST Sequence Type: